The Clean Blood & Tissue DNA Kit is based upon our proprietary magnetic beads based system to extract high quality genomic DNA. It can be used for the genomic DNA isolation from 1-200 μL of fresh or frozen whole blood, buffy coat and saliva or up to 10 mg tissue. The presence of anticoagulants such as Citrate, EDTA and Heparin does not affect the isolation efficiency. The removal of all heating steps for the extraction of DNA from blood allows the Clean Blood & Tissue DNA Kit to be easily implemented on liquid handling workstations (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf). Our Clean Blood & Tissue DNA Kit enables the isolation of high quality DNA, suitable for direct use in most downstream applications, such as PCR, qPCR and Next Generation Sequencing.
Genomic DNA is isolated from the lysed blood or tissue samples in one step by binding on CleanNA particles surfaces. The CleanNA magnetic particles are separated from the lysates by using a magnetic device. Following a few rapid wash steps to remove trace contaminants, the purified DNA is eluted from the CleanNA particles for downstream applications using an Elution Buffer.
Features and benefits
There are several features and benefits that come with the clean blood and tissue DNA kit. First of all there is a high quality DNA yield. That comes with excellent 260/280 and 260/230 ratios. The benefits are: there are no heating steps required (blood protocol), it is a fast and efficient protocol, a scalable solution for isolation from various sample volumes and it is adaptable to many automated liquid handling workstations on the market.
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